## Error in get(paste0(generic, ".", class), envir = get_method_env()) : 
##   object 'type_sum.accel' not found
## 
## Attaching package: 'dplyr'
## The following objects are masked from 'package:stats':
## 
##     filter, lag
## The following objects are masked from 'package:base':
## 
##     intersect, setdiff, setequal, union
# library(rjson)
# library(readxl)

Introduction

This document describes the process of saving and exporting VPR data in a format which is easy to produce, and meets international data expectations of being, open source and accessible. It will describe the process of saving data outputs, cover recommended formats, metadata and data naming suggestions, and more. Note that this is specific to data produced and shared by researchers at Bedford Institute of Oceanography (BIO), but the references may be helpful to a wider audience.

Core Concepts

  • FAIR A set of modern data sharing principles published by Wilkinson, et.al in 2016. Findable, Accessible, Interoperable, Reproducible. These principles mean that data should be shared widely and in formats which are straight-forward for others to access. These principles guide all the decisions made in this vignette.
  • Open Source formats Open source file formats do not require proprietary software in order to download, open or manipulate the files
  • DarwinCore A data and metadata vocabulary which sets standard names for variables relevant to biological diversity. (https://dwc.tdwg.org/)
  • British Oceanographic Data Centre Vocabularies (BODC::P01) A data vocabulary which sets standard names for oceanographic variables. (https://www.bodc.ac.uk/resources/vocabularies/parameter_codes/)

Data products

VPR data can be represented in many forms. In this document we will focus on two formats of final data products, which include environmental & abundance data, in wide and long tables. In both forms, abundance has been calculated by classification category and some relevant metadata has been added.

The long format of data looks like

data(category_conc_n)
head(category_conc_n)
##                    depth  min_depth max_depth depth_diff min_time_s max_time_s
## bad_image_blurry.1     1  0.7012599  1.333086  0.6318261   22454.28   22455.81
## bad_image_blurry.2    51 50.1900481 51.374062  1.1840139   22516.03   22517.43
## bad_image_blurry.3    53 52.2655387 53.817433  1.5518939   22519.79   22521.84
## bad_image_blurry.4    55 55.0559644 55.839339  0.7833749   22522.60   22524.81
## bad_image_blurry.5    57 56.7109645 57.928655  1.2176907   22525.94   22527.83
## bad_image_blurry.6     4  3.8206964  4.339441  0.5187443   22458.24   22459.92
##                    time_diff_s n_roi_bin conc_m3 temperature salinity  density
## bad_image_blurry.1       1.528         0       0  16.8446000 27.09709 19.49410
## bad_image_blurry.2       1.401         0       0   0.4194333 31.98277 25.65109
## bad_image_blurry.3       2.048         0       0   0.4773000 32.04410 25.69756
## bad_image_blurry.4       2.202         0       0   0.4776000 32.07135 25.71949
## bad_image_blurry.5       1.889         0       0   0.4716500 32.08685 25.73228
## bad_image_blurry.6       1.679         0       0  16.8155333 27.09840 19.50157
##                    fluorescence turbidity  time_hr n_frames vol_sampled_bin_m3
## bad_image_blurry.1     85.20000  40.20000 6.237459       10        0.000836630
## bad_image_blurry.2     54.33333  23.66667 6.254662        3        0.000250989
## bad_image_blurry.3     70.00000  40.00000 6.255783        2        0.000167326
## bad_image_blurry.4     52.00000  46.50000 6.256585        2        0.000167326
## bad_image_blurry.5     53.50000  48.50000 6.257468        2        0.000167326
## bad_image_blurry.6     68.00000  48.00000 6.238705        3        0.000250989
##                     time_ms        towyo max_cast_depth         category
## bad_image_blurry.1 22454852  ascending_1       57.92866 bad_image_blurry
## bad_image_blurry.2 22516784  ascending_1       57.92866 bad_image_blurry
## bad_image_blurry.3 22520818  ascending_1       57.92866 bad_image_blurry
## bad_image_blurry.4 22523706  ascending_1       57.92866 bad_image_blurry
## bad_image_blurry.5 22526884  ascending_1       57.92866 bad_image_blurry
## bad_image_blurry.6 22459337 descending_1       43.71157 bad_image_blurry
##                    station
## bad_image_blurry.1    test
## bad_image_blurry.2    test
## bad_image_blurry.3    test
## bad_image_blurry.4    test
## bad_image_blurry.5    test
## bad_image_blurry.6    test

The wide format looks like

data(ctd_roi_merge)
head(ctd_roi_merge)
##    time_ms conductivity temperature pressure salinity fluor_ref fluorescence_mv
## 1 22454281      3.55600     16.8443    0.707  27.0973       695              87
## 2 22454359      3.55600     16.8444    0.780  27.0973       695              87
## 3 22454433      3.55600     16.8444    0.780  27.0973       695              87
## 4 22454510      3.55602     16.8447    0.857  27.0972       695              87
## 5 22454584      3.55602     16.8447    0.857  27.0972       695              87
## 6 22454659      3.55601     16.8449    0.949  27.0970       695              87
##   turbidity_ref turbidity_mv altitude_NA  day hour station   sigmaT     depth
## 1           700           37           0 d222  h03    test 19.49432 0.7012599
## 2           700           37           0 d222  h03    test 19.49430 0.7736670
## 3           700           37           0 d222  h03    test 19.49430 0.7736670
## 4           700           37           0 d222  h03    test 19.49416 0.8500417
## 5           700           37           0 d222  h03    test 19.49416 0.8500417
## 6           700           37           0 d222  h03    test 19.49396 0.9412945
##    roi bad_image_blurry bad_image_malfunction bad_image_strobe Calanus
## 1 <NA>                0                     0                0       0
## 2 <NA>                0                     0                0       0
## 3 <NA>                0                     0                0       0
## 4 <NA>                0                     0                0       0
## 5 <NA>                0                     0                0       0
## 6 <NA>                0                     0                0       0
##   chaetognaths ctenophores krill marine_snow Other small_copepod stick
## 1            0           0     0           0     0             0     0
## 2            0           0     0           0     0             0     0
## 3            0           0     0           0     0             0     0
## 4            0           0     0           0     0             0     0
## 5            0           0     0           0     0             0     0
## 6            0           0     0           0     0             0     0
##   n_roi_total
## 1           0
## 2           0
## 3           0
## 4           0
## 5           0
## 6           0

Reccommended formats

During data processing and for internal work purposes, .Rdata files make a great storage option. They are easy to export with save(). You can capture the data in a variety of formats but most relevant might be a data frame or an oce object. The advantage of the oce object is that it contains a metadata slot which can be useful for preserving self-contained metadata.

When considering sharing the data, more accessible and generic formats should be considered. During a review of file format options, researchers at BIO have decided to utilize a combination of .csv files (for data storage) and .json files (for metadata storage). This combination of files has the advantage of maintaining very simple file formats while capturing a wealth of associated metadata. The files are both very portable and easily accessed through a simple text editor, by users who are not data-experts.

Metadata conventions

Metadata can be stored at a data set level, representing a ‘cruise’ or series of VPR deployments, as well as at an event (station, or cast) level, representing a single VPR deployment. Each level has unique requirements but it should be ensured that the metadata files have a connection (in this example: dataset_ID).

# vpr_metadata_template <- system.file("extdata", "vpr_metadata_template.xlsx",
#                                      package = "vprr")
# 
# metadata_temp_ds <- readxl::read_xlsx(vpr_metadata_template, sheet = 1)
# 
# metadata_temp_st <- readxl::read_xlsx(vpr_metadata_template, sheet = 2)

The recommended format for preserving metadata is JSON A simple, easy to read, file with a well documented structure. To generate a json file from R is very straightforward and can be done through rjson::toJSON(). This function will accept a nested list object and format it into a JSON string which can then be written to an external file.

Data conventions

Columns in VPR data outputs (from vprr) should be renamed for standardization. This example uses a combination of DarwinCore (DwC) and British Oceanographic Data Centre (BODC) P01 vocabularies. Since VPR data contains a unique mix of biological and oceanographic environmental data, it was necessary to use both vocabularies to capture the full scope of the data. Data column names, as well as units and definitions should be included in the metadata (shown in the example below in the ‘dataAttributes’ field).

Example using package data

data(category_conc_n)
metadata <- list("station_level" = list(
     "title" = list("en" = "VPR data from the Scotian Shelf",
                     "fr" = "Données VPR de l'étagère néo-écossaise"),
     "dataset_ID" = 1,
     "decimalLatitudeStart" = 44.5,
     "decimalLongitudeStart" = -64.5,
     "decimalLatitudeEnd" = 45.5,
     "decimalLongitudeEnd" = -65.5,
     "maximumDepthInMeters" = 1000,
     "eventDate" = "2019-08-11",
     "eventTime" = "00:00:00",
     "basisOfRecord" = "MachineObservation",
     "associatedMedia" = "https://ecotaxa.obs-vlfr.fr/ipt/archive.do?r=iml2018051",
     "identificationReferences" = "Iv3 model v3.3",
     "instrument" = list( "opticalSetting" = "S2",
                           "imageVolume" = 83663),
     "resources" = list("data" = list("name" = "vpr123_station25.csv",
                         "creationDate" = "2023-01-01"),
                         "metadata" = list("name" = "vpr123_station25-metadata.json",
                         "creationDate" = "2023-01-01")),
     "dataAttributes" = list("eventID" = list(
                                   "dataType" = "chr",
                                   "definition" = "An identifier for the set of information associated with a dwc:Event (something that occurs at a place and time). May be a global unique identifier or an identifier specific to the data set.",
                                    "vocabulary" = "dwc"),
                              "minimumDepthInMeters" = list(
                                    "dataType" = "float",
                                    "definition" = "The lesser depth of a range of depth below the local",
                                    "vocabulary" = "dwc"),
                              "maximumDepthInMeters" = list(
                                    "dataType" = "float",
                                    "definition" = "The greater depth of a range of depth below the local",
                                    "vocabulary" = "dwc"),
                              "DEPHPRST" = list(
                                    "dataType" = "float",
                                    "definition" = "Depth (spatial coordinate) of sampling event start relative to water surface in the water body by profiling pressure sensor and conversion to depth using unspecified algorithm",
                                    "vocabulary" = "BODC::P01"),
                               "individualCount" = list(
                                    "dataType" = "float",
                                    "definition" = "The number of individuals present at the time of the dwc:Occurrence.",
                                    "vocabulary" = "dwc"),
                               "verbatimIdentification" = list(
                                    "dataType" = "chr",
                                    "definition" = "A string representing the taxonomic identification as it appeared in the original record.",
                                    "vocabulary" = "dwc"),
                               "SDBIOL01" = list(
                                    "dataType" = "float",
                                    "definition" = "Abundance of biological entity specified elsewhere per unit volume of the water body",
                                    "vocabulary" = "BODC::P01"),
                               "TEMPST01" = list(
                                    "dataType" = "float",
                                    "definition" = "Temperature of the water body by CTD or STD",
                                    "vocabulary" = "BODC::P01"),
                               "PSALST01" = list(
                                    "dataType" = "float",
                                    "definition" = "Practical salinity of the water body by CTD and computation using UNESCO 1983 algorithm",
                                    "vocabulary" = "BODC::P01"),
                               "POTDENS0" = list(
                                    "dataType" = "float",
                                    "definition" = "Density (potential) of the water body by computation from salinity and potential temperature using UNESCO algorithm with 0 decibar reference pressure",
                                    "vocabulary" = "BODC::P01"),
                               "FLUOZZZZ" = list(
                                    "dataType" = "float",
                                    "definition" = "Fluorescence of the water body",
                                    "vocabulary" = "BODC::P01"),
                               "TURBXXXX" = list(
                                    "dataType" = "float",
                                    "definition" = "Turbidity of water in the water body",
                                    "vocabulary" = "BODC::P01"),
                               "sampleSizeValue" = list(
                                    "dataType" = "float",
                                    "definition" = "A numeric value for a measurement of the size (time duration, length, area, or volume) of a sample in a sampling dwc:Event.",
                                    "vocabulary" = "dwc"),
                               "sampleSizeUnit" = list(
                                    "dataType" = "chr",
                                    "definition" = "The unit of measurement of the size (time duration, length, area, or volume) of a sample in a sampling dwc:Event.",
                                    "vocabulary" = "dwc"),
                               "scientificName" = list(
                                    "dataType" = "chr",
                                    "definition" = "The full scientific name, with authorship and date information if known. When forming part of a dwc:Identification, this should be the name in lowest level taxonomic rank that can be determined. This term should not contain identification qualifications, which should instead be supplied in the dwc:identificationQualifier term.",
                                    "vocabulary" = "dwc"),
                               "identifiedBy" = list(
                                    "dataType" = "chr",
                                    "definition" = "A list (concatenated and separated) of names of people, groups, or organisations who assigned the Taxon to the subject.",
                                    "vocabulary" = "dwc"),
                               "identificationVerificationStatus" = list(
                                    "dataType" = "chr",
                                    "definition" = "A categorical indicator of the extent to which the taxonomic identification has been verified to be correct.",
                                    "vocabulary" = "dwc"),
                               "depthDifferenceMeters" = list(
                                    "dataType" = "float",
                                    "definition" = "Difference between maximumDepthInMeters and minimumDepthInMeters of an individual data bin, in meters",
                                    "vocabulary" = "BIO"),
                               "minimumTimeSeconds" = list(
                                    "dataType" = "float",
                                    "definition" = "minimum time value in a data bin, measured in seconds from the start of the day of sampling",
                                    "vocabulary" = "BIO"),
                               "maximumTimeSeconds" = list(
                                    "dataType" = "float",
                                    "definition" = "maximum time value in a data bin, measured in seconds from the start of the day of sampling",
                                    "vocabulary" = "BIO"),
                               "timeDifferenceSeconds" = list(
                                    "dataType" = "float",
                                    "definition" = "Difference between maximumTimeSeconds and minimumTimeSeconds of an individual data bin, in seconds",
                                    "vocabulary" = "BIO"),
                               "numberOfFrames" = list(
                                    "dataType" = "float",
                                    "definition" = "number of VPR frames captured within an individual data bin",
                                    "vocabulary" = "BIO"),
                               "timeMilliseconds" = list(
                                    "dataType" = "float",
                                    "definition" = "Time measured in milliseconds since the start of the sampling day",
                                    "vocabulary" = "BIO"),
                               "towyoID" = list(
                                    "dataType" = "chr",
                                    "definition" = "A string identifying the section of the cast to which the data point belongs",
                                    "vocabulary" = "BIO"),
                               "maximumCastDepthInMeters" = list(
                                    "dataType" = "float",
                                    "definition" = "Maximum depth in Meters of the cast dataset",
                                    "vocabulary" = "BIO")
   )))

   # new_name = old_name
columnNames = list( "DEPHPRST" = "depth" ,
   "verbatimIdentification" = "category",
   "eventID" = "station",
   "minimumDepthInMeters" = "min_depth",
   "maximumDepthInMeters" = "max_depth",
   "individualCount" = "n_roi_bin",
   "SDBIOL01" = "conc_m3",
   "TEMPST01" = "temperature",
   "PSALST01" = "salinity",
   "POTDENS0" = "density",
   "FLUOZZZZ" = "fluorescence",
   "TURBXXXX" = "turbidity",
   "sampleSizeValue" = "vol_sampled_bin_m3",
   "depthDifferenceMeters" = "depth_diff",
   "minimumTimeSeconds" = "min_time_s",
   "maximumTimeSeconds" = "max_time_s",
   "timeDifferenceSeconds" = "time_diff_s",
   "numberOfFrames" = "n_frames",
   "timeMilliseconds" = "time_ms",
   "towyoID" = "towyo",
   "maximumCastDepthInMeters" = "max_cast_depth"
   )

# add any new data columns required
# (eg. sampleSizeUnit, scientificName, identifiedBy, identificationVerificationStatus)
sampleSizeUnit <- "cubic metre"
identifiedBy <- "K. Sorochan"
identificationVerificationStatus <- "ValidatedByHuman"

data <- category_conc_n %>%
  dplyr::mutate(., identifiedBy = identifiedBy,
   sampleSizeUnit = sampleSizeUnit,
   identificationVerificationStatus = identificationVerificationStatus)

 # Define the mapping between category and scientific name
 # scientific names based ecotaxa taxonomic system
scientificName <- list("blurry" = "bad_image_blurry",
                       "artefact" = c("bad_image_malfunction", "bad_image_strobe"),
                       "Calanus" = "Calanus")

# Create a new column of data called scientificName based on matches to category
data <- data %>%
  dplyr::mutate(., scientificName = case_when(
    category %in% scientificName[["blurry"]] ~ "blurry",
    category %in% scientificName[["artefact"]] ~ "artefact",
    category == scientificName[["Calanus"]] ~ "Calanus",
    TRUE ~ NA
  ))

# vpr_export(data, metadata, columnNames, file = "vpr123_station25")

Example of outputs

Here are the outputs from the above example.

# data <- read.csv(system.file(
#   "extdata", "vpr123_station25.csv", package = "vprr"))
# head(data)
# 
# metadata <- rjson::fromJSON(file = system.file(
#   "extdata", "vpr123_station25-metadata.json", package = "vprr"))

Conclusion

This document has summarized some of the reasoning behind creating and sharing generic, easily accessible versions of VPR data products, as well as some methodology which could be used to produce standardized data and metadata products. Hopefully this will encourage wide spread sharing of VPR data products and help future research aims.

References

Wilkinson, M., Dumontier, M., Aalbersberg, I. et al. The FAIR Guiding Principles for scientific data management and stewardship. Sci Data 3, 160018 (2016). https://doi.org/10.1038/sdata.2016.18